Genetic differentiation measures were moderate, with a mean F ST of 0.074, G ST of 0.081 and R ST of 0.085. ResultsThe mean expected heterozygosity across loci within populations ranged from 0.62 to 0.77. The aim of this work is to study the genetic status of this breed through the analysis of molecular data from 10 microsatellites typed in historic and actual live animals. Guadarrama goat is a threatened Spanish breed which actual census (2008) consists of 3057 females and 203 males distributed in 22 populations more or less isolated. Analysis of microsatellite loci is known to be highly informative in the reconstruction of the historical processes underlying the evolution and differentiation of animal populations. BackgroundAssessing genetic biodiversity and population structure of minor breeds through the information provided by neutral molecular markers, allows determination of their extinction risk and to design strategies for their management and conservation.
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